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BioSpec - Research project

Quantitative analysis of biomarkers by spectrometry

Post-doctoral fellow: Arthur GAUTHERON

ABSTRACT

Spectrophotometric analysis has many advantages for in vivo characterization of human biological tissues and classification of healthy or pathological tissues: it is fast and contactless. The search for spectral patterns allows for example to detect the presence of biological components, chromophores or fluorophores, characteristic of some tumors. The challenge of the project is to be able not only to detect but also to quantify these components, i.e. to estimate their concentration in a reliable way, in order for example to help the surgeon to decide the perimeter of the tumor area to be resected. The envisaged methodology includes a rigorous photometric analysis of the measurement system and an optical model for light scattering and fluorescence in the various layers of tissues, which allow, in an inverse approach, to deduce the concentrations of components of interest from the spectral measurements. The difficulty of this approach is mainly due to the translucency of biological materials (light trajectories are more complex to describe than for more opaque materials), and to the constraints related to in vivo measurements (reflection measurements are possible, but not transmission measurements, which limits the data available for evaluating the parameters of the model). Applications in dermatology (melanoma and other cancers), neurosurgery (gliomas) and organ transplantation (liver, kidney) are targeted.