Projets en cours, Research

2 new publications in the LASIMP project!

On The September 26, 2022

Inscription setup and the scanning direction employed for texturization - Steve PAPA / SAINBIOSE lab
Inscription setup and the scanning direction employed for texturization - Steve PAPA / SAINBIOSE lab

Steve PAPA, our PhD student on the LASIMP project, published 2 new scientific articles with his team

Steve PAPA, PhD student at the SAINBIOSE lab, published two new articles in the Applied Physics and Applied Surface Science reviews.

Pallarés Aldeiturriaga, David & Papa, Steve & Abou Khalil, Alain & Pascale Hamri, Alina & Maalouf, Mathieu & Di Maio, Yoan & Guignandon, Alain & Dumas, Virginie & Sedao, Xxx. (2022). Influence of multi-wavelength ultrafast laser texturing and autoclave sterilization on titanium alloy-based surface wettability. Applied Physics A. 128. 10.1007/s00339-022-06015-9.


ABSTRACT

Titanium alloys are widely used in bio-medical applications for their excellent bio-compatibility and superior mechanical strength. On the other hand, femtosecond laser processing is a robust versatile and industrial method widely used for surface functionalization. In this work, laser-induced periodic surface structures (LIPSS) on titanium alloy (Ti6Al4V) using different laser wavelengths of 1030 nm (IR), 515 nm (Green) and 257 nm (UV) were created and their surface wettability (physical parameter correlated with cell adhesion) was studied, in relation to sterilization process. The contact angle over LIPSS was smaller than over polished samples, with Green and UV LIPSS presenting contact angles inferior to 20∘ and a superior stability to sterilization process.



 

Papa, Steve & Abou Khalil, Alain & Hamzeh-Cognasse, Hind & Thomas, Mireille & Maalouf, Mathieu & Di Maio, Yoan & Sedao, Xxx & Guignandon, Alain & Dumas, Virginie. (2022). Dual-functionalized titanium by ultrafast laser texturing to enhance human gingival fibroblasts adhesion and minimize Porphyromonas gingivalis colonization. Applied Surface Science. 606. 154784. 10.1016/j.apsusc.2022.154784.


ABSTRACT

Dental implant failure is primarily due to peri-implantitis, a consequence of bacterial penetration causing implant loosening. Beyond supporting the implant, the adhesion of gingival tissues to the surface is essential for its role as a physical barrier to bacteria. Surface nanotextures are widely known to affect wettability, protein adsorption, and cellular and/or bacterial adhesion. In this report, a femtosecond laser (fs-L) was used to design Laser-Induced Periodic Surface Structures (LIPSS) with Infra-Red (IR) or Green lasers on Ti6Al4V. Surface morphology, topography and wettability were characterized. Gingival fibroblasts adhesion was evaluated with sclerometer scratch tests and confirmed by ultrasonic bath cell detachment method. The fs-L texturing increases human gingival cells adhesion compared to polished surface. The adhesion of peri-implantitis associated bacteria was quantified. After 48h of contact, IR LIPSS show antiadhesive properties for Porphyromonas gingivalis, whereas Green LIPSS, thanks to its smaller spatial period, have limited both Streptococcus mutans and Porphyromonas gingivalis adhesion. Thus, LIPSS generated on Ti6Al4V surfaces, especially Green LIPSS, are identified as having double biological functionality: repellent for bacteria and adhesive for gingival tissues. This study proves that fs-L processing is an innovative tool with potential to enhance implant success paving the way towards dual functionality.